Publications

2021

  1. Zhengjie Zhou, Chih-Fan Yeh, Michael Mellas, Myung-Jin Oh, Jiayu Zhu, Jin Li, Ru-Ting Huang, Devin Harrison, Tzu-Pin Shentu, David Wu, Michael Lueckheide, Lauryn Carver, Eun Ji Chung, Lorraine Leon, Kai-Chien Yang, Matthew V. Tirrell, Yun Fang, Targeted polyelectrolyte complex micelles treat vascular complications in vivo, PNAS, 2021, doi: 10.1073/pnas.2114842118.
  2. Jin Li, Yun Fang* and David Wu*. Mechanical forces and metabolic changes cooperate to drive cellular memory and endothelial phenotypes. Current Topics in Membranes: Cellular Mechanostransduction Mechanisms to Cardiovascular and Fibrotic Diseases. Accepted. 2021.
  3. Devin Harrison*, David Wu*, Jun Huang, Yun Fang. Single-cell lactate production rate as a measure of glycolysis in endothelial cells. STAR Protocols. 2021. doi: 10.1016/j.xpro.2021.100807. *equivalent contribution (co-first authorship)
  4. Rengul Cetin-Atalay, Angelo Y. Meliton, David Wu, Lucas M. Kimmig, Parker S. Woods, Robert B. Hamanaka, Ying-Jie Peng, Jayasri Nanduri, Nanduri Prabhakar, and Gökhan M. Mutlu. Intermittent hypoxia-induced activation of endothelial cells is mediated via sympathetic activation dependent catecholamine release. Frontiers in Physiology. 2021. DOI: 10.3389/fphys.2021.701995
  5. David Wu*, Devin Harrison*, Teodora Szasz, Chih-Fan Yeh, Tzu-Pin Shentu, Angelo Meliton, Ru-Ting Huang, Zhengjie Zhou, Gökhan Mutlu, Jun Huang, Yun Fang. Single-cell metabolic imaging reveals a RhoA-triggered glycolytic burst in motile endothelial cells. Nature Metabolism. 3, 714–727 (2021). doi.org/10.1038/s42255-021-00390-yPreprint available on Research Squares. 2021. doi: 21203/rs.3.rs-149025/v1 *equivalent contribution (co-first authorship)
    Accompanying editorial: Subramanian, A., Becker, L.M. & Carmeliet, P. Endothelial metabolism going single. Nature Metabolism 3, 593–594 (2021). doi.org/10.1038/s42255-021-00399-3.
  6. David Wu*, Tzu-Han Lee*, Ru-Ting Huang, Rob Guzy, Nathan Schoettler, Ayodeji Adegunsoye, Jeffrey Mueller, Anne Sperling, Aliya Hussein, Gökhan M. Mutlu, Yun Fang#. SARS-CoV-2 reduces Krüppel-Like Factor 2 in lung autopsy samples. American Journal of Respiratory Cell and Molecular Biology. 2021. doi: 10.1165/rcmb.2020-0564LE  * equivalent contribution (co-first authorship
  7. Phillip McMullen, Peter Pytel, Alexis Snyder, Heather Smith, Jasmine Vickery, James Brainer, Robert Guzy, David Wu, Nathan Schoettler, Ayodeji Adegunsoye, Anne Sperling, John Hart, Lindsay Alpert, Anthony Chang, Sandeep Gurbuxani, Thomas Krausz, Aliya N. Husain, Jeffrey Mueller. A Series of COVID-19 Autopsies with Clinical and Pathologic Comparisons to both Seasonal and Pandemic Influenza. Journal of Pathology: Clinical Research. doi:10.1002/cjp2.220. 2021.
  8. Robinder G. Khemani, Rémi Coudroy, David Wu, Edward J Schenck, Jessica T. Lee, Margaret M. Hayes, Patricia A. Kritek, Gökhan M. Mutlu, Hayley B. Gershengorn. Update in Critical Care 2020. American Journal of Respiratory and Critical Care Medicine. doi: 10.1164/rccm.202102-0336UP2021.

2020

  1. Lucas M Kimmig*, David Wu*, Matthew Gold, Natasha N Pettit, David Pitrak, Jeffrey Mueller, Aliya N Husain, Ece A Mutlu, Gökhan M Mutlu. IL6 inhibition in critically ill COVID-19 patients is associated with increased secondary infections. Frontiers in Medicine. 2020. doi: 10.3389/fmed.2020.583897 * equivalent contribution (co-first authorship)
  2. Natasha N. Pettit, Cynthia T. Nguyen, Gökhan M. Mutlu, David Wu, Lucas Kimmig, David Pitrak, Kenneth Pursell. Late Onset Infectious Complications and Safety of Tocilizumab in the Management of COVID-19. Journal of Medical Virology. 2020. doi: 10.1002/jmv.26429
  3. Youjin V. Lee, David Wu*, Yuxing Peng*, Yun Fang, and Bozhi Tian*. A sensitive tool for probing cellular mechanics: tracking longitudinal rotation of a kinked-silicon nanowire. Nano Letters  2020, 20, 5, 3852–385. doi.org/10.1021/acs.nanolett.0c00974. * equivalent contribution (co-corresponding authorship)

2019

  1. Yun Fang, David Wu, Yun Fang, Konstantin Birukov. Mechanotransduction in endothelial cells. Comprehensive Physiology. 2019. Mar 15;9(2):873-904. doi: 10.1002/cphy.c180020
  2. David Wu, Konstatin Birukov. Endothelial cell mechano-metabolomic coupling to disease states in the lung microvasculature. Frontiers in Bioengineering and Biotechnology. 2019, July doi: 10.3389/fbioe.2019.00172

2018

  1. Matthew Krause, Ru-Ting Huang, David Wu, Tzu-Pin Shentu, Devin L. Harrison, Michael B. Whalen, Lindsay K. Stolze, Ivan P. Moskowitz, Mete Civelek, Casey E. Romanoski, Yun Fang. A genetic variant at coronary artery disease and ischemic stroke locus 1p32.2 regulates endothelial responses to hemodynamics. Proceedings of the National Academy of Sciences U.S.A doi: 10.1101/377549.
  2. Yi-Ju Chen, David Wu, William Gelbart, Charles Knobler, Rob Phillips, Willem K. Kegel. Two-stage dynamics of invivo bacteriophage genome ejection. Physical Review X 8, 021029, 2018
  3. David Wu, Parker S Woods, Heng T Duong. Role of cellular metabolism in pulmonary diseases. American Journal of Respiratory Cell and Molecular Biology. 2018; Jul;59(1):127-129. doi: 10.1165/rcmb.2018-0103RO.
  4. David Wu. Cardiovascular Disorders. In: ATS Review for the Critical Care Boards. 2018.  American Thoracic Society, New York, NY.

2017

  1. Robert B. Hamanaka, Recep Nigdelioglu, Angelo Y. Meliton, Yufeng Tian, Leah J. Witt, Erin O’Leary, Kaitlyn A. Sun, Parker S. Woods, David Wu, Brandon Ansbro, Shawn Ard, Jason M. Rohde, Nickolai O. Dulin, Robert D. Guzy, and Gökhan M. Mutlu. Inhibition of PHGDH attenuates bleomycin-induced pulmonary fibrosis. American Journal of Respiratory Cell and Molecular Biology. 2017; Oct 11. doi: 10.1165/rcmb.2017-0186OC
  2. David Wu, Ru-Ting Huang, Robert B. Hamanaka, Matthew Krause, Myung-Jin Oh, Cheng-Hsiang Kuo, RecepNigdelioglu, Angelo Y. Meliton, Leah J. Witt, Guohao Dai, Mete Civelek, Nanduri R. Prabhakar, Yun Fang*, Gökhan M. Mutlu*. HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcinevascular endothelium. eLife 2017;10.7554/eLife.25217.* equivalent contribution (cocorresponding authorship)
  3. Ru-TingHuang*, David Wu*, Angelo Meliton, Myung-Jin Oh, Joyce A. Lloyd, Recep Nigdelioglu, Robert B. Hamanaka, Mukesh K. Jain, Anna Birukova, John P. Kress, Konstantin G. Birukov, Gökhan M. Mutlu, Yun Fang. Experimental lung injury reduces KLF2 to increase endothelial permeability via regulation of RAPGEF3-Rac1 signaling. American Journal of Respiratory and Critical Care Medicine. doi: 10.1164/rccm.201604-0668OC. Mar1;195(5):639-651, 2017.* equivalent contribution (cofirst authorship)
  4. David Wu, Robert B. Hamanaka, Yun Fang, Gökhan M. Mutlu. Letter by Wu et al Regarding Article, “Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.” Arterioscler Thromb Vasc Biol. 2017 Dec;37(12):e197-e198. doi: 10.1161/ATVBAHA.117.310335

2016

  1. Recep Nigdelioglu, Robert B. Hamanaka, Angelo Y. Meliton, Erin O’Leary, Leah J. Witt, Takugo Cho, Kaitlyn Sun, Catherine Bonham, David Wu, Parker S. Woods, Aliya N. Husain, Don Wolfgeher, Nickolai O. Dulin, Navdeep S. Chandel, and Gökhan M. Mutlu. TGF-β promotes de novo serine synthesis for collagen production. The Journal of Biological Chemistry, pii: jbc.M756247, 2016.

Before 2016

  1. Halina Anton, Sebastien Harlepp, Caroline Ramspacher, David Wu, Fabien Monduc, Sandeep Bhat, MichaelLiebling, Camille Paoletti, Gilles Charvin, Jonathan B Freund, and Julien Vermot. Pulse propagation by acapacitive mechanism drives embryonic blood flow. Development, 140(21): 4426-34, 2013.
  2. David Van Valen*, David Wu*, Yi-Ju Chen, Hannah Tuson, Paul Wiggins, Rob Phillips. A single-moleculeHershey-Chase experiment. Current Biology, 22: 1399, 2012.* equivalent contribution (cofirst authorshipFeatured in EditorsChoice Science, 337: 624, 2012.
  3. Weihong C. Qiu, Nathan D. Derr, Brian S. Goodman, Elizabeth Villa, David Wu, William Shih, and Samara L.Reck-Peterson. Dynein achieves processive motion using both stochastic and coordinated stepping. Nature Structural Molecular Biology, 19(2): 193-200, 2012.
  4. Le A. Trinh, Tatiana Hochgreb, Matthew Graham, David Wu, Frederique Ruf, Chathurani Jayasena, Ankur Saxena, Rasheeda Hawk, Aidyl Gonzalez-Serricchio, Alana Dixson, Elly Chow, Constanza Gonzales, Ho-YinLeung, Ilana Solomon, Marianne Bronner-Fraser, Sean G. Megason, and Scott E. Fraser. A versatile gene trap tovisualize and interrogate the function of the vertebrate proteome. Genes and Development, 25(21): 2306-2320, 2011.
  5. David Wu, Jonathan Freund, Scott Fraser, Julien Vermot. Mechanistic basis of otolith formation during teleostinner ear development. Developmental Cell. 20(2): 271-278, 2011. Featured as cover article.
  6. Periklis Pantazis, James Maloney, David Wu, Scott Fraser. Second harmonic generating (SHG) nanoprobes for invivo imaging. Proceedings of the National Academy of Sciences U.S.A. 107(33): 14535-14540, 2010.
  7. David D. Wu*, David A. Van Valen*, Qicong Hu, Rob Phillips. Ion-dependent dynamics of DNA ejections forbacteriophage lambda. Biophysical Journal. 99(4): 1101-1109, 2010.* equivalent contribution (cofirst authorship)
  8. Julien Vermot, Arian S. Forouhar, Michael Liebling, David D. Wu, Diane Plummer, Morteza Gharib, Scott E.Fraser. Reversing blood flow acts through klf2a to ensure normal valvulogenesis in the developing heart. PLoSBiology. 7(11):e1000246, 2009.
  9. David D. Wu, Kingshuk Ghosh, Mandar Inamdar, Heun-jin Lee, Scott Fraser, Ken Dill, Rob Phillips. A trajectoryapproach to two-state kinetics of single particles on sculpted energy landscapes. Physical Review Letters. 103: 050603, 2009.
  10. Jessica R. Colantonio*, Julien Vermot*, David D. Wu, Adam D. Langenbacher, Scott Fraser, Jaunian Chen, KentL. Hill. The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear. Nature.457:205-209, 2009.* equivalent contribution (cofirst authorship)
  11. Shaohua Xu, David D. Wu, Morton Arnsdorf, Robert Johnson, Godfrey S. Getz, Veneracion G. Cabana. Chemicalcolloids versus biological colloids: A comparative study for the elucidation of the mechanism of protein fiberformation. Biochemistry. 44: 5381-5389, 2005.
  12. Rajan P. Kulkarni, David D. Wu, Mark E. Davis, Scott E. Fraser. Quantitating intracellular transport ofpolyplexes by spatio-temporal image correlation spectroscopy. Proceedings of the National Academy ofSciences U.S.A. 102: 7523-7528, 2005.
  13. Xiaohui Qu, David Wu, Laurens Mets, Norbert F. Scherer. Nanometer-localized multiple single-moleculefluorescence microscopy. Proceedings of the National Academy of Sciences U.S.A. 101: 11298-11303, 2004.